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Enhanced production of

Shen Huang,Xudong Feng,Chun Li

Frontiers of Chemical Science and Engineering 2015, Volume 9, Issue 4,   Pages 501-510 doi: 10.1007/s11705-015-1544-0

Abstract: -Glucuronidase from Li-3 (PGUS) can efficiently hydrolyze glycyrrhizin into the more valuable glycyrrhetic acid monoglucuronide. However, a low productivity of PGUS and the lack of an effective separation strategy have significantly limited its industrial applications. Therefore, the production of PGUS has been improved by optimizing both the fermentation and purification strategies. A two-stage fermentation strategy was developed where PGUS was first grown with glucose and then PGUS was produced in the presence of glycyrrhizin as an inducer. By using this strategy, the biomass was increased 1.5 times and the PGUS activity increased 5.4 times compared to that when glycyrrhizin was used as the sole carbon source. The amount of PGUS produced was increased another 16.6% when the fermentation was expanded to a 15-L fermenter. An effective protocol was also established to purify the PGUS using a sequential combination of hydrophobic, strong anion-exchange and gel filtration chromatography. This protocol had a recovery yield of 6% and gave PGUS that was 39 times purer than the crude PGUS. The purified PGUS had a specific activity of 350 U·mg .

Keywords： β-glucuronidase     glycyrrhetic acid monoglucuronide     cell disruption     purification     chromatography    

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Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization

Wan N. A. Kadir, Man K. Lam, Yoshimitsu Uemura, Jun W. Lim, Peck L. Kiew, Steven Lim, Siti S. Rosli, Chung Y. Wong, Pau L. Show, Keat T. Lee

Frontiers of Chemical Science and Engineering 2021, Volume 15, Issue 5,   Pages 1257-1268 doi: 10.1007/s11705-020-2015-9

Abstract: In the present study, ozone was introduced as an alternative approach to harvest and disrupt microalgae cells ( ) simultaneously for biodiesel production. At the optimum ozonation conditions (6.14 g·h ozone concentration, 30 min ozonation time, 1 L·min of ozone flowrate at medium pH of 10 and temperature of 30 °C), the sedimentation efficiency of microalgae cells increased significantly from 12.56% to 68.62%. It was observed that the microalgae cells aggregated to form flocs after pre-treated with ozone due to the increment of surface charge from –20 to –6.59 mV. Besides, ozone had successfully disrupted the microalgae cells and resulted in efficient lipid extraction, which was 1.9 times higher than the control sample. The extracted microalgae lipid was mainly consisted of methyl palmitate (C16:0), methyl oleate (C18:1) and methyl linolenate (C18:3), making it suitable for biodiesel production. Finally, utilization of recycled culture media after ozonation pre-treatment showed robust growth of microalgae, in which the biomass yield was maintained in the range of 0.796 to 0.879 g·h for 5 cycles of cultivation.

Keywords： microalgae     biodiesel     ozonation     pre-treatment     lipid    

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Two-stage stochastic programming with robust constraints for the logistics network post-disruption response

Frontiers of Engineering Management   Pages 67-81 doi: 10.1007/s42524-022-0240-2

Abstract: To address the LN post-disruption response strategy optimization problem, this study proposes a novel

Keywords： logistics network design     post-disruption response strategy     two-stage stochastic programming     conditional    

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Modeling of the resilient supply chain system from a perspective of production design changes

Frontiers of Engineering Management   Pages 96-106 doi: 10.1007/s42524-022-0235-z

Abstract: To overcome the impact of insufficient raw material supply on the supply chain in mass disruption scenarios

Keywords： resilient supply chain     supply chain disruption     domain modeling     product design changes    

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Evaluation of endocrine disruption and dioxin-like effects of organic extracts from sewage sludge in

LIU Cao,XU Yiping,MA Mei,HUANG Bingbin,WU Jingdong,MENG Qingyi,WANG Zijian,GEARHEART Robert Alan

Frontiers of Environmental Science & Engineering 2014, Volume 8, Issue 3,   Pages 433-440 doi: 10.1007/s11783-013-0594-0

Abstract: based on a batch of in vitro bioassays, including a set of recombinant gene yeast assays for endocrine disruption

Keywords： sewage sludge     in vitro bioassay     endocrine disruption     dioxin-like effect    

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CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells

Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 554-562 doi: 10.1007/s11684-017-0543-6

Abstract: with chimeric antigen receptor (CAR) have been successfully applied to treat advanced refractory B cellOne negative regulator of T cell activity is lymphocyte activation gene-3 (LAG-3).nbsp;LAG-3 knockout CAR-T cells displayed robust antigen-specific antitumor activity in cell

Keywords： CAR-T     CRISPR-Cas9     LAG-3    

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Airline Disruption Management: A Review of Models and Solution Methods Review

Yi Su, Kexin Xie, Hongjian Wang, Zhe Liang, Wanpracha Art Chaovalitwongse, Panos M. Pardalos

Engineering 2021, Volume 7, Issue 4,   Pages 435-447 doi: 10.1016/j.eng.2020.08.021

Abstract: unpredictable factors, such as severe weather, airport closure, and corrective maintenance, leading to disruptionWhen a disruption occurs, the airline operation control center performs various operations to reassignWe introduce different sources of disruption and corresponding operations.recovery, and integrated recovery are reviewed, with the aim of providing models and methods for different disruption

Keywords： Disruption management     Aircraft recovery problem     Crew recovery problem     Integrated recovery problem    

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Metro train rescheduling by adding backup trains under disrupted scenarios

Jiateng YIN, Yihui WANG, Tao TANG, Jing XUN, Shuai SU

Frontiers of Engineering Management 2017, Volume 4, Issue 4,   Pages 418-427 doi: 10.15302/J-FEM-2017044

Abstract: aim of train rescheduling is to frequently dispatch trains to evacuate delayed passengers after the disruption

Keywords： train rescheduling     backup train     metro line     disruption     timetable    

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Omnichannel retail operations with ship-to-store and ship-from-store options under supply disruption

Frontiers of Engineering Management   Pages 158-170 doi: 10.1007/s42524-022-0238-9

Abstract: recent years, but few studies have explored omnichannel retailing operations in response to supply disruptionfulfillment options (i.e., ship-from-store and ship-to-store options) can mitigate the risk of supply disruptionInterestingly, it is possible to achieve a win–win–win outcome when the supply disruption risk is highentire supply chain benefits from the omnichannel retailing strategy even if it faces a high level of disruption

Keywords： supply chain disruption     omnichannel fulfillment option     ship-from-store     ship-to-store    

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Microfluidics for cell-cell interactions: A review

Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng

Frontiers of Chemical Science and Engineering 2016, Volume 10, Issue 1,   Pages 90-98 doi: 10.1007/s11705-015-1550-2

Abstract: The well-designed microscale intermediary is also ideal for the study of cell biology.Particularly, microfluidic chip is helpful for better understanding cell-cell interactions.

Keywords： microfluidic chip     co-culture     cell-cell interactions     review    

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Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in

Frontiers of Medicine   Pages 939-956 doi: 10.1007/s11684-023-0987-9

Abstract: 645 cardiac cells were generated from embryonic day 17.5 and postnatal days 2 and 8 mice by single-cellMNDCMs having interactions with macrophages and non-proliferative MNDCMs (non-pMNDCMs) with minimal cell–cellThese MNDCMs were mapped to the heart by integrating the spatial and single-cell transcriptomic data.In conclusion, a novel non-pMNDCM subpopulation with minimal cell–cell communications was unveiled, highlighting

Keywords： mononuclear diploid cardiomyocytes     cell–cell communication     cardiac fibroblast     single-cell RNA sequencing    

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Deubiquitinases as pivotal regulators of T cell functions

Xiao-Dong Yang, Shao-Cong Sun

Frontiers of Medicine 2018, Volume 12, Issue 4,   Pages 451-462 doi: 10.1007/s11684-018-0651-y

Abstract: Defect in T cell activation is associated with severe immune deficiencies, whereas aberrant T cell activationAn emerging mechanism that regulates T cell activation and tolerance is ubiquitination, a reversibleIt is now well recognized that DUBs are crucial regulators of T cell responses and serve as potential

Keywords： deubiquitinase     ubiquitination     T cell activation     T cell differentiation     T cell tolerance    

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Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 46-57 doi: 10.1007/s11705-017-1609-3

Abstract: Cell surface protein engineering facilitated by accumulation of information on genome and protein structureinvolves heterologous production and modification of cell surface proteins using genetic engineering, and is important for the development of high-performance whole-cell catalysts.In this field, cell surface display is a major technology by exposing target proteins, such as enzymes, on the cell surface using a carrier protein.

Keywords： cell surface engineering     surface display     whole-cell catalysts     bioprocess    

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Stem cell niches and endogenous electric fields in tissue repair

Li LI, Jianxin JIANG

Frontiers of Medicine 2011, Volume 5, Issue 1,   Pages 40-44 doi: 10.1007/s11684-011-0108-z

Abstract: Endogenous adult stem cells reside in certain regions of organs, known as the stem cell niche, whichMany regulatory factors are involved in the stem cell-based repair process, including stem cell nichesHere we briefly review the role of stem cell niches and endogenous electric fields in tissue repair,and hypothesize that endogenous electric fields become part of stem cell niche in the wound site.

Keywords： stem cell     stem cell niche     electric field     tissue repair    

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CAR T-cell immunotherapy: a powerful weapon for fighting hematological B-cell malignancies

Frontiers of Medicine 2021, Volume 15, Issue 6,   Pages 783-804 doi: 10.1007/s11684-021-0904-z

Abstract: Chimeric antigen receptor T-cell (CAR T) immunotherapy opens a new path for targeted therapy of hematologicalIn this review, through a representative case study, we summarize the current experience of CAR T-cellthe causative mechanisms of therapy resistance, and new strategies to improve the efficacy of CAR T-cell

Keywords： CAR T cells     hematological malignancies     review    

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Title Author Date Type Operation

Enhanced production of

Shen Huang,Xudong Feng,Chun Li

Journal Article

Simultaneous harvesting and cell disruption of microalgae using ozone bubbles: optimization and characterization

Wan N. A. Kadir, Man K. Lam, Yoshimitsu Uemura, Jun W. Lim, Peck L. Kiew, Steven Lim, Siti S. Rosli, Chung Y. Wong, Pau L. Show, Keat T. Lee

Journal Article

Two-stage stochastic programming with robust constraints for the logistics network post-disruption response

Journal Article

Modeling of the resilient supply chain system from a perspective of production design changes

Journal Article

Evaluation of endocrine disruption and dioxin-like effects of organic extracts from sewage sludge in

LIU Cao,XU Yiping,MA Mei,HUANG Bingbin,WU Jingdong,MENG Qingyi,WANG Zijian,GEARHEART Robert Alan

Journal Article

CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells

Yongping Zhang, Xingying Zhang, Chen Cheng, Wei Mu, Xiaojuan Liu, Na Li, Xiaofei Wei, Xiang Liu, Changqing Xia, Haoyi Wang

Journal Article

Airline Disruption Management: A Review of Models and Solution Methods

Yi Su, Kexin Xie, Hongjian Wang, Zhe Liang, Wanpracha Art Chaovalitwongse, Panos M. Pardalos

Journal Article

Metro train rescheduling by adding backup trains under disrupted scenarios

Jiateng YIN, Yihui WANG, Tao TANG, Jing XUN, Shuai SU

Journal Article

Omnichannel retail operations with ship-to-store and ship-from-store options under supply disruption

Journal Article

Microfluidics for cell-cell interactions: A review

Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng

Journal Article

Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in

Journal Article

Deubiquitinases as pivotal regulators of T cell functions

Xiao-Dong Yang, Shao-Cong Sun

Journal Article

Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Journal Article

Stem cell niches and endogenous electric fields in tissue repair

Li LI, Jianxin JIANG

Journal Article

CAR T-cell immunotherapy: a powerful weapon for fighting hematological B-cell malignancies

Journal Article